Quin Tran Kim do the extraction on an anthill with a weight of 2-3 kg using some type of solvent such as water, methanol and methanol-water mixture. Several types of advanced cancer such as cervical cancer, lung cancer and colon cancer that has grown on media containing extracts of the ant nest. From the results of culture were anthill provide exceptional results, extract the ant nest can inhibit cancer cell growth (antiproliferasi) with activities like this then extract ant nests are able to control the growth and development of cancer cells despite being in an advanced stage or metastatic.
In Biology Pharmaceutical Bulletin, Qui Kim Tran and colleagues stated that the cancer cells in humans can be controlled with ant nest extract. At the level of effectiveness of the methanol extract of EC50 reached 9.97 mg / nl, which means to get the effect that is able to suppress cancer cell growth by 50% then the required number of doses of ant extract only 9.97 mg / nl. For the water extract of the ant nest, EC50 is 22.3 mg / nl while the water-methanol extract 11.3 mg / nl. This is the basis why the ant nest can control the growth and development of cancer cells.
Tocopherol compounds also contained quite a lot in the ant nest. Tocopherol is Vitamin E which has antioxidant activity that is strong enough. Professor of Pharmacy Institute of Technology Bandung, Prof. Dr. Elin Yulinan Sukandar, the research states that the content of tocopherol in the ant nest is quite high. Tocopherol functions as an antioxidant and anticancer so tocopherol may also function in preventing the occurrence of cancer. Tocopherol as an antioxidant works by counteracting free radical attacks originating from outside our bodies. Besides as an antioxidant, tocopherol is also useful as anti-aging or antiaging. According to Ahmad Sulaiman PhD who is an expert on nutrition graduate of the University of Nebraska Lincoln said that vitamin E is very vital role for our health. Methods that vitamin E is to prevent the formation of unsaturated fatty acids.
Other research on the effects of the ant nest by Muhammad Ahkam Subroto which is a doctoral graduate of the University of New South Wales, Sydney, he saw in terms of consumer safety by measuring the LD50 is the amount of dose required to cause the death of as many as 50% of a given population. Apparently, a dose 3 times daily tablespoon or 3 x 15 ml is still very safe to be consumed by patients.
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